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Postdoctoral Scientist - PNAC - Dr Jason Chin and Dr Julian Sale - LMB 2066 Open Date 08/02/2023, 11:00 Close Date 08/03/2023, 23:55 Band MRC - 4 Location Cambridge Salary £35,143 per annum Contract Type Fixed Term Job Type Science Full Time / Part Time Full Time Contract Length 2 years Job Description Overall purpose: To work within the groups of Jason Chin and Julian Sale at the MRC Laboratory of Molecular Biology (LMB), on a Wellcome Trust-funded programme, entitled ‘Human Genome Synthesis’, aimed at creating a synthetic, human chromosome. Specifically, to undertake research for creating human cells harbouring a synthetic genome. The project, is a collaboration between the laboratories of Jason Chin and Julian Sale at LMB, Gianluca Petris at the Wellcome Sanger Institute in Hinxton, Tom Ellis at Imperial College London and Patrick Cai at the University of Manchester. This LMB post will focus on the convergent assembly of large synthetic DNAs, transferring large DNAs between different hosts and cells, integrating episomal DNA into chromosomes within host cells, strategies for identifying deleterious synthetic DNA sequences, and computational analysis and prediction allowed synthetic replacements from genomic sequence. It is anticipated that individual posts will focus on a subset of these goals within the overall strategy of the project. Main duties: • To undertake research aimed at addressing the specific project areas described above. • To identify, develop and apply a broad range of techniques to pursue the research objectives. • To present scientific work at seminars within the laboratory and at external meetings. • To contribute to laboratory-wide discussions on developments within the field. • To draft scientific papers, and contribute to the overall preparation of research for publication. • To contribute to the LMB’s mission in the public engagement of science, and the translation of research findings into improvements in health care. • To assist in the training of PhD students and other members of the LMB. Key responsibilities: Within the overall direction of the programme, the group, the remit of the project and in discussion with the Group Leader, you will make a significant input into determining the direction of the project within a 2-year lifespan. To plan your own work and objectives on a 12-month basis and manage your experimental work within the project along with your Supervisor. To work with limited supervision to identify, develop, modify and apply the necessary techniques to achieve the goals of the project. To introduce and apply new techniques across a wide range of disciplines and to have the creativity and initiative to develop novel approaches and methods where required. To ensure the research is carried out in accordance with good practice and in compliance with local policies and legal requirements. To contribute to the smooth running of the group, including the effective use of resources, training of others and taking responsibility for use of communal facilities. To enhance your research and generic skills through a tailored development programme. Working relationships: You will report to Dr. Jason Chin and / or Dr. Julian Sale and will interact and collaborate with other Postdoctoral Scientists, Research Support staff and students, not only in your group, but also across the LMB and with external groups as necessary. Additional information:
Academic qualifications: These should include a PhD in a relevant subject or due to complete PhD within 6 months. Technical skills and expertise: Experience of and ability to perform techniques relevant to the project. Particularly useful would be experience of: • Core molecular biology techniques • Culture and genetic manipulation of E. coli, BACs, recombineering • Knowledge of bacterial replication and recombination mechanisms • Culture and genetic manipulation of vertebrate cells (e.g. mammalian, animal etc.), cell transfection & fusion techniques; CRISPR genome editing; chromosome transfer • Flow cytometry. Cell and chromosome sorting • Knowledge of mechanisms of mammalian replication, recombination and chromosome segregation • Analysis of genomic deep sequencing data Track record of research: This will include achievements, and contributions to published papers. Other relevant evidence of: • Commitment. • Originality. • Ability to communicate. • Working with others.
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发表于 2023-2-20 14:07:27